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An Organic Sol‐Gel Film as Modifier to Construct Biosensor
Author(s) -
Wu JianFeng,
Xu MiaoQing,
Zhao GuangChao
Publication year - 2009
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200804460
Subject(s) - biosensor , sol gel , hydrogen peroxide , adsorption , amperometry , resorcinol , formaldehyde , chemistry , detection limit , immobilized enzyme , monomer , inorganic chemistry , nuclear chemistry , materials science , electrode , chromatography , electrochemistry , nanotechnology , polymer , organic chemistry , enzyme
A new amperometric biosensor for hydrogen peroxide (H 2 O 2 ) was developed by adsorbing hemoglobin (Hb) on an organic sol‐gel film. The organic sol‐gel was prepared using resorcinol and formaldehyde as monomers. This sol‐gel film shows a biocompatible microenvironment for retaining the native activity of the adsorbed Hb. The direct electron transfer between Hb and electrode is achieved. Hb adsorbed on the film shows an enzyme‐like catalytic activity for the reduction of H 2 O 2 . The reduction peak currents are proportional linearly to the concentration of hydrogen peroxide in the range of 6×10 −8 to 3.6×10 −6 M, with a detection limit of 2.4×10 −8 M ( S/N =3). This research enlarges the applications of organic sol‐gel materials in biosensor field.