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Electrochemical Methods for the Investigation of Bioaffinity Interactions Based on Gold Nanoparticles Modified Sensors
Author(s) -
Shumyantseva Victoria,
Suprun Elena,
Bulko Tatiana,
Archakov Alexander
Publication year - 2009
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200804439
Subject(s) - colloidal gold , myoglobin , electrochemistry , electrode , nanoparticle , surface modification , bromide , materials science , membrane , graphite , nanotechnology , chemistry , combinatorial chemistry , inorganic chemistry , organic chemistry , biochemistry
Gold nanoparticles (AuNP) stabilized by the synthetic membrane‐like compound didodecyldimethylammonium bromide (DDAB) were used for the modification of graphite screen‐printed electrodes (SPE). Two different electrochemical approaches based on AuNP modified sensors for the detection of bioaffinity interactions were developed. As a model human cardiac myoglobin–complementary antimyoglobin antibody couple was taken. In the first method AuNP worked as an electrochemical sensing platform: the oxidation of the gold surface (resulted in gold oxide formation) upon polarization served as a basis for analytical response. In the second assay, AuNP served as effective electrocatalysts of Fe 3+ /Fe 2+ electrode reaction of myoglobin. For both assays the same AuNP synthesis and electrodes modification procedures were used.