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Novel Composite of Multiwalled Carbon Nanotubes and Gold Nanoparticles Stabilized by Chitosan and Hydrophilic Ionic Liquid for Direct Electron Transfer of Glucose Oxidase
Author(s) -
Li Jiangwen,
Zhao Faqiong,
Wang Guangyin,
Gui Zhe,
Xiao Fei,
Zeng Baizhao
Publication year - 2009
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200804413
Subject(s) - ionic liquid , x ray photoelectron spectroscopy , glucose oxidase , colloidal gold , materials science , nanoparticle , electron transfer , carbon nanotube , electrochemistry , chitosan , chemical engineering , tetrafluoroborate , transmission electron microscopy , electrode , analytical chemistry (journal) , catalysis , chemistry , nanotechnology , photochemistry , organic chemistry , biosensor , engineering
A novel composite was fabricated through dispersing multiwalled carbon nanotubes (MWNTs) in gold nanoparticle (GPs) colloid stabilized by chitosan and ionic liquid (i.e., 1‐butyl‐3‐methylimidazolium tetrafluoroborate, BMIMBF 4 ). Transmission electron microscopy (TEM) experiment showed that the GPs highly dispersed on the MWNTs probably due to the electrostatic interaction among GPs, MWNTs and the imidazolium cation of BMIMBF 4 . X‐ray photoelectron spectroscopy (XPS) indicated that thus‐formed gold nanostructure was mediated by BMIMBF 4 . When glucose oxidase (GOD) was immobilized on the composite (MWNTs‐GPs) its ultraviolet‐visible absorption spectrum kept almost unchanged. The immobilized GOD coated glassy carbon electrode (GOD/MWNTs‐GPs/GC) exhibited a pair of well‐defined peaks in 0.10 M pH 7.0 phosphate buffer solution (PBS), with a formal potential of −0.463 V (vs. SCE). The electrochemical process involved two‐electron transfer. The electron transfer coefficient was ca.0.56 and the electron transfer rate constant was 9.36 s −1 . Furthermore, the immobilized GOD presented good catalytic activity to the oxidation of glucose in air‐saturated PBS. The K m and I m values were estimated to be 13.7 μM and 0.619 μA. The GOD/MWNTs‐GPs/GC electrode displayed good stability and reproducibility.