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Genosensor for SARS Virus Detection Based on Gold Nanostructured Screen‐Printed Carbon Electrodes
Author(s) -
MartínezParedes Graciela,
GonzálezGarcía María Begoña,
CostaGarcía Agustín
Publication year - 2009
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200804399
Subject(s) - colloidal gold , electrode , detection limit , biotinylation , materials science , nanotechnology , carbon fibers , covid-19 , nanoparticle , chemistry , chromatography , composite number , composite material , biochemistry , medicine , disease , pathology , infectious disease (medical specialty)
A hybridization‐based genosensor is designed on a disposable gold nanostructured screen‐printed carbon electrode. Gold nanoparticles which are formed in situ by applying a constant current intensity during a fixed time, acts as an immobilization and transduction surface. The parameters affecting the methodology are studied, with special attention being placed on sensibility. Immobilization takes place through thiol‐gold interaction in a relatively fast way, and the genosensor response is found to be linearly to the biotinylated target concentration between 2.5 and 50 pmol/L; a detection limit of 2.5 pmol/L is calculated, and a sensitivity of 1.76 μA/pmol L −1 is achieved. These results improve significantly those described in literature, thus demonstrating the advantages offered by the use of gold‐nanostructured surfaces.