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An Electrochemical Immunosensor for C‐Reactive Protein Based on Multi‐Walled Carbon Nanotube‐Modified Electrodes
Author(s) -
Buch Michal,
Rishpon Judith
Publication year - 2008
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200804358
Subject(s) - c reactive protein , carbon nanotube , biomarker , immunoassay , electrode , asymptomatic , materials science , medicine , electrochemistry , inflammation , antibody , nanotechnology , chemistry , immunology , biochemistry
C‐reactive protein (CRP) is a nonspecific biomarker of inflammation and infection that can be used as a predictive risk marker of cardiovascular disease in asymptomatic individuals or as a prognostic marker of recurrent ischemia and death among patients with coronary heart disease or stroke. We developed a sensitive, disposable, and easy to operate sensor based on heterogeneous sandwich immunoassay for high sensitivity CRP (hs‐CRP) measurement. We used screen‐printed electrodes modified with multi‐walled carbon nanotubes and protein A to ensure the oriented immobilization of anti‐CRP antibodies. CRP was quantitatively measured down to a concentration of 0.5 ng mL −1 , enabling high dilution of the samples before measurement and consequently reducing interferences present in the serum. The sensor developed is suitable for point of care detection of hs‐CRP at clinically relevant concentrations for the diagnosis of both conventional and low‐grade inflammations.