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Amperometric Detection and Quantification of 8‐Hydroxy‐2′‐deoxyguanosine (8‐OHdG) Using Dendrimer Modified Electrodes
Author(s) -
Gutiérrez Alejandro,
Osegueda Soraya,
GutiérrezGranados Silvia,
Alatorre Alejandro,
García Ma. Guadalupe,
Godínez Luis A.
Publication year - 2008
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200804324
Subject(s) - dendrimer , amperometry , chemistry , electrode , thiol , detection limit , chromatography , matrix (chemical analysis) , high performance liquid chromatography , deoxyguanosine , combinatorial chemistry , electrochemistry , organic chemistry , adduct
Abstract 8‐Hydroxy‐2′‐deoxyguanosine (8‐OHdG) detection by high performance liquid chromatography (HPLC) with amperometric detection was studied using a Au electrode modified with different dendrimer based thin films. Gold electrode is thiol‐modified, forming self‐assembled monolayers on which different generation PAMAM dendrimers with terminal functional groups COOH and NH 2 have been attached using peptidic bonds. Results obtained in synthetic samples show low limits of detection and quantification for 8‐OHdG (1.2×10 −9 and 3.7×10 −9 M respectively), with matrix interference elimination, thus avoiding sample pretreatment. Best results are obtained with electrodes modified with aliphatic amino thiols and 3.5 and 4.5 generation carboxylated dendrimers (Au/AET/DG 3.5 and Au/AET/DG 4.5 ), demonstrating that these materials constitute a good alternative for 8‐OHdG determination in biological fluids.