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Comparison of Electrochemical and Surface Plasmon Resonance Immunosensor Responses on Single Thin Film
Author(s) -
Kurita Ryoji,
Nakamoto Kohei,
Ueda Akio,
Niwa Osamu
Publication year - 2008
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200804318
Subject(s) - surface plasmon resonance , materials science , electrochemistry , colloidal gold , detection limit , electrode , nanometre , analytical chemistry (journal) , layer (electronics) , alkaline phosphatase , nanotechnology , chemical engineering , nuclear chemistry , chromatography , chemistry , nanoparticle , composite material , enzyme , organic chemistry , engineering
This paper reports results obtained when comparing an electrochemical enzyme immunosensor and a surface plasmon resonance (SPR) based immunosensor on the same gold surface installed in an electrochemical SPR flow cell. Simultaneous electrochemical and SPR measurements were performed on a gold surface modified with multilayers of poly‐ L ‐lysine and poly‐styrenesulfonate assembled with the layer‐by‐layer method. First, we obtained the SPR response induced by the formation of an immunocomplex from the shift in the SPR angle by injecting an anti tumor necrosis factor‐α antibody solution labeled with alkaline phosphatase into the flow cell containing the multilayer modified with tumor necrosis factor‐α. Then we compared this SPR result with that obtained for the electrochemical oxidation current of p ‐aminophenol catalyzed by alkaline phosphatase from p ‐aminophenolphosphate on the same gold film. We compared the two immunosensor responses obtained using the different measurement principles and found that there was a high correlation efficient of 0.973 between them. This was because we were able to immobilize the immunoreagents with good stability and without losing the transport of the enzyme product in the multilayer whose thickness we easily controlled with nanometer scale accuracy. We also report that the detection limit of our electrochemical immunosensor after optimization was around 100 pg/mL (0.4 pM), which is one of the lowest values yet reported for an electrochemical immunosensor.