Premium
Multilayer Assembly of Hemoglobin and Colloidal Gold Nanoparticles on Multiwall Carbon Nanotubes/Chitosan Composite for Detecting Hydrogen Peroxide
Author(s) -
Chen Shihong,
Yuan Ruo,
Chai Yaqin,
Yin Bing,
Xu Yang
Publication year - 2008
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200804303
Subject(s) - colloidal gold , carbon nanotube , hydrogen peroxide , cyclic voltammetry , materials science , adsorption , chitosan , nanoparticle , electrochemistry , chemical engineering , biosensor , colloid , nanotechnology , nuclear chemistry , electrode , chemistry , organic chemistry , engineering
Chitosan (CS) was chosen for dispersing multi‐wall carbon nanotubes (MWNTs) to form a stable CS‐MWNTs composite, which was first coated on the surface of a glassy carbon electrode to provide a containing amino groups interface for assembling colloidal gold nanoparticles (GNPs), followed by the adsorption of hemoglobin (Hb). Repeating the assembly step of GNPs and Hb resulted in {Hb/GNPs} n multilayers. The assembly of GNPs onto CS‐MWNTs composites was confirmed by transmission electron microscopy. The consecutive growth of {Hb/GNPs} n multilayers was confirmed by cyclic voltammetry and UV‐vis absorption spectroscopy. The resulting system brings a new platform for electrochemical devices by using the synergistic action of the electrocatalytic activity of GNPs and MWNTs. The resulting biosensor displays an excellent electrocatalytic activity and rapid response for hydrogen peroxide. The linear range for the determination of H 2 O 2 was from 5.0×10 −7 to 2.0×10 −3 M with a detection limit of 2.1×10 −7 M at 3 σ and a Michaelis–Menten constant K M app value of 0.19 mM.