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Dendritic Silver/Silicon Dioxide Nanocomposite Modified Electrodes for Electrochemical Sensing of Hydrogen Peroxide
Author(s) -
Yuan Peixi,
Zhuo Ying,
Chai Yaqin,
Ju Huangxian
Publication year - 2008
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200804266
Subject(s) - hydrogen peroxide , horseradish peroxidase , biosensor , amperometry , electrochemistry , nanocomposite , detection limit , electrode , linear range , chemistry , nuclear chemistry , inorganic chemistry , silicon dioxide , materials science , chemical engineering , chromatography , nanotechnology , organic chemistry , enzyme , engineering , metallurgy
A novel biosensor for hydrogen peroxide was prepared by immobilizing horseradish peroxidase (HPR) on newly synthesized dendritic silver/silicon dioxide nanocomposites, which were coated on a glassy carbon electrode. The modified electrode was characterized with XPS, SEM, and electrochemical methods. This biosensor showed a very fast amperometric response to hydrogen peroxide with a linear range from 0.7 to 120 μM, a limit of detection of 0.05 μM and a sensitivity of 1.02 mA mM −1 cm −2 . The Michaelis‐Menten constant of the immobilized HRP was estimated to be 0.21 mM, indicating a high affinity of the HRP to H 2 O 2 without loss of enzymatic activity. The preparation of the proposed biosensor was convenient, and it showed high sensitivity and good stability.