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Selective Electrochemical Determination of Uric Acid in the Presence of Ascorbic Acid Using a Carbon Paste Electrode Modified with β‐Cyclodextrin
Author(s) -
RamírezBerriozabal Minerva,
Galicia Laura,
GutiérrezGranados Silvia,
Cortes José Sandoval,
Herrasti Pilar
Publication year - 2008
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200804232
Subject(s) - ascorbic acid , detection limit , amperometry , chemistry , carbon paste electrode , selectivity , uric acid , electrochemistry , electrode , supporting electrolyte , cyclodextrin , chromatography , electrolyte , nuclear chemistry , inorganic chemistry , carbon fibers , cyclic voltammetry , organic chemistry , materials science , biochemistry , catalysis , food science , composite material , composite number
Uric acid (UA) was determined in the presence of ascorbic acid (AA) by using a carbon paste electrode modified superficially by a β‐cyclodextrin film (CPE/β‐CD). The surface carbon paste electrode was prepared applying a 30 cycles potential program and using a 1 M HClO 4 +0.01 M β‐CD electrolytic solution. The UA and AA solutions were used to evaluate the electrode selectivity and sensitivity by cyclic voltammetric and amperometric methods. In these experiments the detection limit for UA was (4.6±0.01)×10 −6 M and the RSD calculated from the amperometric curves was 10%. From the data obtained it was possible to quantify UA in the urine and saliva samples. Selective detection of UA was improved by formation of an inclusion complex between β‐CD and UA. The results show that the CPE/β‐CD is a good candidate due to its selectivity and sensitivity in the UA determination in complex samples like the biological fluids.