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Hemin Modified Carbon Fat Electrode for Analyzing Antimalarial Endoperoxide Artemisinin in Artemisia annua
Author(s) -
Debnath Chhanda,
Saha Pijus,
Ortner Astrid
Publication year - 2008
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200804222
Subject(s) - artemisinin , hemin , artemisia annua , differential pulse voltammetry , chemistry , cyclic voltammetry , detection limit , carbon paste electrode , electrode , electrochemistry , catalysis , voltammetry , inorganic chemistry , chromatography , organic chemistry , plasmodium falciparum , heme , malaria , immunology , biology , enzyme
A hemin bulk modified carbon electrode with Adeps neutralis (solid fat) as binder was developed for the determination of antimalarial endoperoxide artemisinin in plant matrix. The hemin modified electrode showed significant catalytic activity for the electrochemical reduction of artemisinin at about −380 mV vs. Ag/AgCl in phosphate buffer solution of pH 7 by using cyclic and differential pulse voltammetry. Under optimized conditions strict linearity between artemisinin concentration and height of the cathodic catalytic current peak was observed in 4.8×10 −6 –7.8×10 −5 M concentration range ( R =0.9991) when using differential pulse voltammetry. The detection limit was calculated as 1.4×10 −6 M of artemisinin. The developed electroanalytical device is suitable for the determination of artemisinin in Artemisia annua extracts.