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Constant Current Chronopotentiometry and Voltammetry of Native and Denatured Serum Albumin at Mercury and Carbon Electrodes
Author(s) -
Ostatná Veronika,
Kuralay Filiz,
Trnková Libuše,
Paleček Emil
Publication year - 2008
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200804206
Subject(s) - bovine serum albumin , chemistry , voltammetry , electrode , electrochemistry , analytical chemistry (journal) , dropping mercury electrode , cyclic voltammetry , mercury (programming language) , albumin , chromatography , inorganic chemistry , biochemistry , computer science , programming language
Constant current chronopotentiometric peak H at mercury electrodes was recently shown as a sensitive tool for global and local changes in protein conformation [1]. Large differences between the heights of peak H of native (hBSA nat ) and denatured BSA (hBSA den ) were observed. The ratio hBSA den /hBSA nat increased with more negative stripping current suggesting that the rate of potential change is important for discrimination between native and denatured BSA. Voltammetric peaks of BSA were less well developed and BSA den /BSA nat was much smaller. It was not possible to discriminate BSA den and BSA nat using carbon electrodes.