Direct Electrochemical Monitoring of RNase Activity | Zendy

Ye Yongkang | Zendy; Wen Wen | Zendy; Xiang Yun | Zendy; Qi Xiaodong | Zendy; La Belle Jeffrey T. | Zendy; Chen Julian J. L. | Zendy; Wang Joseph | Zendy

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Direct Electrochemical Monitoring of RNase Activity

Author(s) -

Ye Yongkang,

Wen Wen,

Xiang Yun,

Qi Xiaodong,

La Belle Jeffrey T.,

Chen Julian J. L.,

Wang Joseph

Publication year - 2008

Publication title -

electroanalysis

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.574

H-Index - 128

eISSN - 1521-4109

pISSN - 1040-0397

DOI - 10.1002/elan.200804172

Subject(s) - detection limit , rnase p , chemistry , electrochemistry , ribonuclease , potentiometric titration , chromatography , rna , combinatorial chemistry , biochemistry , electrode , gene

Here we present a highly sensitive, rapid and simple electrochemical assay of RNase based on coupling magnetic separation of the enzymatically treated RNA with stripping potentiometric detection of the purine nucleobases. A detection limit of 1×10 −8 U RNase (ca. 4 pg/mL) is obtained in connection to a 60 min enzymatic digestion. The attractive performance of this direct indicator‐free electrochemical assay offers great promise for a wide range of molecular biology and water quality applications.

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