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Direct Electrochemical Monitoring of RNase Activity
Author(s) -
Ye Yongkang,
Wen Wen,
Xiang Yun,
Qi Xiaodong,
La Belle Jeffrey T.,
Chen Julian J. L.,
Wang Joseph
Publication year - 2008
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200804172
Subject(s) - detection limit , rnase p , chemistry , electrochemistry , ribonuclease , potentiometric titration , chromatography , rna , combinatorial chemistry , biochemistry , electrode , gene
Here we present a highly sensitive, rapid and simple electrochemical assay of RNase based on coupling magnetic separation of the enzymatically treated RNA with stripping potentiometric detection of the purine nucleobases. A detection limit of 1×10 −8 U RNase (ca. 4 pg/mL) is obtained in connection to a 60 min enzymatic digestion. The attractive performance of this direct indicator‐free electrochemical assay offers great promise for a wide range of molecular biology and water quality applications.