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Response Behavior of Amperometric Glucose Biosensors Based on Different Carbon Substrate Transducers Coated with Enzyme‐Active Layer: A Comparative Study
Author(s) -
Mauko Lea,
Ogorevc Bozidar,
Pihlar Boris
Publication year - 2009
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200800569
Subject(s) - biosensor , amperometry , substrate (aquarium) , glassy carbon , glucose oxidase , materials science , calibration curve , carbon fibers , electrode , immobilized enzyme , analytical chemistry (journal) , chemistry , chemical engineering , chromatography , nanotechnology , electrochemistry , detection limit , organic chemistry , cyclic voltammetry , enzyme , composite material , oceanography , engineering , geology , composite number
Carbon electrodes (glassy carbon, GC, screen‐printed carbon, SPC, and carbon fiber, CF) were used as substrate transducers to prepare glucose biosensors of different sizes and geometries, based on iron‐ruthenium hexacyanoferrate as H 2 O 2 reduction mediator and glucose oxidase immobilized in a poly(1,2‐phenylenediamine) membrane. Their response behavior under hydrodynamic amperometric conditions at an operating potential of −0.02 V vs. Ag/AgCl was studied and compared. While the GC and SPC based conventional size biosensors showed enzymatic catalysis controlled current response with nonlinear concentration dependence, the CF based micro‐biosensor exhibited, due to diffusion‐controlled current response, extended linear range calibration curves with relatively lower sensitivity and longer response times. Several preparation parameters responsible for the improvement of biosensor performance were also investigated.