Premium
Dual‐Genic Hybridization Sensor Employing Electrochemical Impedance Spectroscopy
Author(s) -
Bonanni Alessandra,
Calvo Daniel,
del Valle Manuel
Publication year - 2008
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200704184
Subject(s) - dielectric spectroscopy , dna–dna hybridization , electrical impedance , dna , materials science , electrode , analytical chemistry (journal) , dual (grammatical number) , electrochemistry , epoxy , chemistry , chromatography , biochemistry , physics , art , literature , quantum mechanics , composite material
A dual‐genic impedimetric sensor for DNA hybridization detection is proposed for the first time. Two different single‐stranded DNA probes (P 1 =d(pA) 20 and P 2 =d(pG) 20 ) were immobilized onto the graphite‐epoxy electrode surface and impedimetric measurements were performed. Hybridization experiments were carried out employing complementary targets (P 1 c =d(pT) 20 or P 2 c =d(pC) 20 ) alone, or combined (P 1 c +P 2 c ), as well as noncomplementary DNA sequences. As from the direct observation of impedance spectra it was not possible to resolve the binary gene mixture, the use of artificial neural networks (ANN) was proposed for extracting significant information to get desired results.