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Polarographic Behavior of Human Plasma Thrombin and Changes in Its Anticoagulation Activity Through Various Conformations
Author(s) -
Singh Niyati,
Pitre K. S.
Publication year - 2008
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200704174
Subject(s) - thrombin , heparin , chemistry , albumin , coagulation , polarography , discovery and development of direct thrombin inhibitors , pharmacology , biochemistry , medicine , platelet , inorganic chemistry
Thrombin plays a pivotal role in the regulation of blood fluidity and therefore constitutes a primary target in the treatment of various hemostatic disorders. For this differential pulse polarography has been developed in an appropriate supporting electrolyte, which produced well‐defined DPP peaks. The average prothrombin time ( PT ) in citrated plasma samples was determined, which indicated decrease of average PT with rise of thrombin and albumin concentrations, while PT resulted differently (increase) with heparin, thus confirming their role in anticoagulation activity. The PT of thrombin‐heparin and thrombin‐albumin conformations showed sharp increased and steady decreased respectively. Thus, two opposite actions of thrombin, coagulation (thrombin‐albumin) and anticoagulation (thrombin‐heparin) may prove fruitful in clinical approach for thrombotic disorders.