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A Novel Electrochemical DNA Biosensor Fabricated with Layer‐by‐Layer Covalent Attachment of Multiwalled Carbon Nanotubes and Gold Nanoparticles
Author(s) -
Ma Haiyan,
Zhang Liping,
Pan Yan,
Zhang Keying,
Zhang Yuzhong
Publication year - 2008
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200704169
Subject(s) - biosensor , differential pulse voltammetry , colloidal gold , cyclic voltammetry , detection limit , carbon nanotube , dielectric spectroscopy , materials science , nanoparticle , covalent bond , nanotechnology , layer by layer , electrochemistry , dna–dna hybridization , layer (electronics) , analytical chemistry (journal) , chemical engineering , dna , electrode , chemistry , chromatography , organic chemistry , biochemistry , engineering
A novel DNA biosensor has been fabricated for the detection of DNA hybridization based on layer‐by‐layer (LBL) covalent assembly of gold nanoparticles (GNPs) and multiwalled carbon nanotubes (MWCNTs). The stepwise LBL assembly process was characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The hybridization events were monitored by differential pulse voltammetry (DPV) measurement of the intercalated doxorubicin, and the factors influencing the performance of the DNA hybridization was investigated in detail. The signal was linearly changed with target DNA concentration increased from 0.5 to 0.01 nM, and had a detection limit of 7.5 pM (signal/noise ratio of 3). In addition, the DNA biosensor showed an excellent reproducibility and stability under the DNA‐hybridization conditions.