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Electrochemical DNA Hybridization Detection Using DNA Cleavage
Author(s) -
Kwon Dohyoung,
Kim Kyuwon,
Kwak Juhyoun
Publication year - 2008
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200704166
Subject(s) - differential pulse voltammetry , dna , nuclease , dna–dna hybridization , hybridization probe , chemistry , single molecule real time sequencing , cleave , nucleic acid thermodynamics , cleavage (geology) , cyclic voltammetry , microbiology and biotechnology , combinatorial chemistry , electrode , dna sequencing , electrochemistry , biochemistry , biology , base sequence , paleontology , fracture (geology) , dna sequencer
We report the new method for detection of DNA hybridization using enzymatic cleavage. The strategy is based on that S1 nuclease is able to specifically cleave only single strand DNA, but not double strand DNA. The capture probe DNA, thiolated single strand DNA labeled with electroactive ferrocene group, was immobilized on a gold electrode. After hybridization of target DNA of complementary and noncomplementary sequences, nonhybridized single strand DNA was cleaved using S1 nuclease. The difference of enzymatic cleavage on the modified gold electrode was characterized by cyclic voltammetry and differential pulse voltammetry. We successfully applied this method to the sequence‐selective discrimination between perfectly matched and mismatched target DNA including a single‐base mismatched target DNA. Our method does not require either hybridization indicators or other exogenous signaling molecules which most of the electrochemical hybridization detection systems require.