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Electrochemical Oxidation of Guanosine and Xanthosine at Physiological pH: Further Evidences of a Convergent Mechanism for the Oxidation of Purine Nucleosides
Author(s) -
GonzálezFernández Eva,
delosSantosÁlvarez Noemí,
LoboCastañón María Jesús,
MirandaOrdieres Arturo J.,
TuñónBlanco Paulino
Publication year - 2008
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200704153
Subject(s) - chemistry , electrochemistry , guanosine , inorganic chemistry , electrolysis , amine gas treating , deoxyguanosine , combinatorial chemistry , electrode , organic chemistry , electrolyte , biochemistry , adduct
The electrochemical oxidation of guanosine and xanthosine, which only differ in the presence of an oxygen atom at C2 position instead of an amine group, is studied on pyrolytic graphite electrodes at different electrolysis time scales in solutions of physiological pH. The oxidation products adsorbed on the electrode surface were electrochemical characterized in terms of variation of the formal potential with pH and catalytic activity towards the oxidation of NADH. The results were compared with those previously described for the oxidation of guanosine and adenosine in alkaline solutions. A common oxidation product is described, the oxidized form of 9‐β‐ D ‐ribofuranosyluric acid, which is proposed as the point of convergence in the oxidation routes of all three purine nucleosides.