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Direct Electrochemistry of Hemoglobin Entrapped in Composite Electrodeposited Chitosan‐Multiwall Carbon Nanotubes and Nanogold Particles Membrane and Its Electrocatalytic Application
Author(s) -
Liu Chuanyin,
Hu Jiming
Publication year - 2008
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200704150
Subject(s) - hydrogen peroxide , chitosan , carbon nanotube , electrochemistry , redox , electrocatalyst , selectivity , biosensor , nanoparticle , composite number , nuclear chemistry , chemistry , nanocomposite , materials science , chemical engineering , electrode , inorganic chemistry , catalysis , nanotechnology , organic chemistry , composite material , engineering
Hemoglobin was entrapped in composite electrodeposited chitosan‐multiwall carbon nanotubes (MCNTs) film by assembling gold nanoparticles and hemoglobin step by step. In phosphate buffer solution (pH 7), a pair of well‐defined and quasireversible redox peaks appeared with formal potential at −0.289 V and peak separation of 100 mV. The redox peaks respected for the direct electrochemistry of hemoglobin at the surface of chitosan‐MCNTs‐gold nanoparticles modified electrode. The parameters of experiments have also been optimized. The composite electrode showed excellent electrocatalysis to peroxide hydrogen and oxygen, the peak current was linearly proportional to H 2 O 2 concentration in the range from 1×10 −6 mol/L to 4.7×10 −4 mol/L with a detection limit of 5.0×10 −7 mol/L, and this biosensor exhibited high stability, good reproducibility and better selectivity. The biosensor showed a Michaelis–Menten kinetic response as H 2 O 2 concentration is larger than 5.0×10 −4 mol/L, the apparent Michaelis–Menten constant for hydrogen peroxide was calculated to be 1.61 μmol/L.