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An Amperometric Biosensor for trans ‐Resveratrol Determination in Aqueous Solutions by Means of Carbon Paste Electrodes Modified with Peroxidase Basic Isoenzymes from Brassica Napus
Author(s) -
Granero Adrián Marcelo,
Fernández Héctor,
Agostini Elizabeth,
Zón María Alicia
Publication year - 2008
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200704109
Subject(s) - biosensor , amperometry , chemistry , chromatography , horseradish peroxidase , repeatability , nuclear chemistry , carbon paste electrode , substrate (aquarium) , calibration curve , ferrocene , detection limit , electrode , analytical chemistry (journal) , cyclic voltammetry , organic chemistry , electrochemistry , biochemistry , enzyme , oceanography , geology
The catalytic properties of peroxidase basic isoenzymes (PBI's) from Brassica napus towards trans‐ resveratrol (t‐Res) oxidation were demonstrated by the first time by conventional UV‐visible spectroscopic measurements. The enzymatic reaction rate was studied under different experimental conditions and the kinetics parameters were determined. An amperometric biosensor based on Brassica napus PBI's to determine t‐Res is also proposed by the first time. The method employs a dialysis membrane covered, PBI's entrapped and ferrocene (Fc)‐embedded carbon paste electrode (PBI's‐Fc‐CP) and is based on the fact that the decreased amount of H 2 O 2 produced by the action of PBI's is proportional to the oxidised amount of t‐Res in the solution. Comparative amperometric experiments showed that, in spite of PBI's activity was much lower than commercial horseradish peroxidase (HRP) activity, t‐Res was a much better substrate for PBI's biosensors than those biosensors constructed by using HRP. The PBI's‐Fc‐CP biosensors showed a very good stability during at least twenty days. The reproducibility and the repeatability were 4.5% and 8.3%, respectively, showing a good biosensor performance. The calibration curve was linear in the t‐Res concentration ( c t‐Res ) range from 1×10 −6 to 2.5×10 −5 M, with a sensibility of (2.31±0.05)×10 6 nA M −1 . The lowest c t‐Res value measured experimentally for a signal to noise ratio of 3 : 1 was 0.83 μM.