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Detecting Biorecognition Events at Blocked Interface Polymeric Membrane Ion‐Selective Electrodes Using Electrochemical Impedance Spectroscopy and Atomic Force Microscopy
Author(s) -
De Marco Roland,
Ng Andrew,
Panduwinata Dwi
Publication year - 2008
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200704064
Subject(s) - potentiometric titration , dielectric spectroscopy , electrode , membrane , biosensor , analytical chemistry (journal) , materials science , electrochemistry , potentiometric sensor , adsorption , chemical engineering , chemistry , nanotechnology , chromatography , biochemistry , engineering
Immobilization of a biorecognition element onto a polymeric membrane ion‐selective electrode (ISE) using a self‐assembly approach may provide scope for a novel biosensor technology platform based on the altered potentiometric response at the blocked ISE interface. In this paper, the authors have investigated the influence of solution adsorption of the model biorecognition element, avidin‐biotin, on the electrode kinetics of a conventional polymeric membrane Ca 2+ ISE using atomic force microscopy (AFM) coupled with electrochemical impedance spectroscopy (EIS). It is demonstrated that solution adsorption of avidin followed by biotin incorporation leads to a demonstrable biorecognition event characterized by an impediment in the Ca 2+ ion transfer kinetics of the modified ISE surface. This kinetic principle is amenable to biosensing using pulsed chronopotentiometric polymeric ISEs, which is an established dynamic electrode technique for use with polymeric membrane ISEs.