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Chronopotentiometric Determination of Redox States of Peptides
Author(s) -
Dorčák Vlastimil,
Paleček Emil
Publication year - 2007
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200704034
Subject(s) - hanging mercury drop electrode , chemistry , redox , electrochemistry , electrode , dropping mercury electrode , peptide , voltammetry , adsorption , drop (telecommunication) , stripping (fiber) , cyclic voltammetry , inorganic chemistry , analytical chemistry (journal) , chromatography , organic chemistry , biochemistry , materials science , telecommunications , composite material , computer science
Reduced and oxidized forms of peptides were studied by different electrochemical methods at carbon and hanging mercury drop (HMDE) electrodes. Striking differences between the reduced and oxidized peptides were obtained by constant current chronopotentiometric stripping analysis (CPSA) at HMDE. Peptides yielded electrocatalytic peak H at highly negative potentials (ca. −1.75 V). Reduced peptides adsorbed at positively charged HMDE produced substantially higher peak H than the oxidized forms. Voltammetry reflected the peptide redox state less efficiently. Different orientation of reduced and oxidized molecules at the positively charged electrode and very fast potential changes in CPSA were probably responsible for the observed effects.