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Preparation of Nanogapped Gold Nanoparticle Array for DNA Detection
Author(s) -
Tokonami Shiho,
Shiigi Hiroshi,
Nagaoka Tsutomu
Publication year - 2008
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200704031
Subject(s) - oligonucleotide , colloidal gold , nanoparticle , materials science , electrode , dna , biosensor , nanotechnology , drop (telecommunication) , analytical chemistry (journal) , chemistry , chromatography , computer science , telecommunications , biochemistry
Abstract A novel DNA detection technique using a gold nanoparticle array film electrode has been reported here. The gold nanoparticles molecularly linked with binder molecule (1,10‐decanedithiol) were separated 1.3 nm from each other, and the DNA conductivity change from single to double strand was measured by monitoring a voltage drop across the particles, between which a probe of a 12‐mer oligonucleotide was immobilized. In adding a complementary oligonucleotide on the nanoparticle film chip, an immediate decrease in the film resistance (ca. 1.4 Ω) due to a hybridization event occurred in a reproducible manner with this simple setup. In the paper, we have an interest in the primary sensing properties; effect of the film resistance on the sensor response, dependence of the resistance change on the DNA concentration, and the performance of the system for DNA detection including single nucleotide polymorphisms were described.