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Electroanalytical Determination of Enrofloxacin in Reversed Phase HPLC
Author(s) -
Sereno Silvia del C.,
Sereno Leónides E.,
Marioli Juan M.
Publication year - 2007
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200704017
Subject(s) - enrofloxacin , detection limit , amperometry , chemistry , chromatography , high performance liquid chromatography , linear range , calibration curve , analytical chemistry (journal) , electroanalytical method , aqueous solution , electrochemistry , electrode , materials science , organic chemistry , potentiometric titration , biochemistry , ciprofloxacin , antibiotics
The electrochemical behavior of a glassy carbon electrode is investigated towards the oxidation of enrofloxacin in aqueous solutions of different pH. Two oxidative waves are observed at a potential range from 0.70 V to 1.20 V (vs. SCE), the intensity of each depending on the pH of the solution. These results are used to set the parameters for the amperometric detection of enrofloxacin in HPLC analysis. A wide linear range and high sensitivity are obtained for a calibration plot using the optimized experimental conditions. The limit of detection ( S / N =3) obtained using those conditions is approximately 0.2 pmol. The applicability of the methodology is demonstrated in the analysis of enrofloxacin in fortified milk samples.