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Amperometric Biosensor for Hydrogen Peroxide, Using Supramolecularly Immobilized Horseradish Peroxidase on the β‐Cyclodextrin‐Coated Gold Electrode
Author(s) -
Camacho Conrado,
Matías Juan C.,
Chico Belkis,
Cao Roberto,
Gómez Leissy,
Simpson Benjamin K.,
Villalonga Reynaldo
Publication year - 2007
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200703993
Subject(s) - horseradish peroxidase , amperometry , biosensor , chemistry , hydrogen peroxide , hydroquinone , detection limit , electrode , cyclodextrin , nuclear chemistry , peroxidase , inorganic chemistry , electrochemistry , chromatography , organic chemistry , enzyme , biochemistry
Horseradish peroxidase, previously modified with 1‐adamantane moieties, was supramolecularly immobilized on gold electrodes coated with perthiolated β‐cyclodextrin. The functionalized electrode was employed for the construction of an amperometric biosensor device for hydrogen peroxide using 1 mM hydroquinone as electrochemical mediator. The biosensor exhibited a fast amperometric response (6 s) and a good linear response toward H 2 O 2 concentration between 12 μM and 450 μM. The biosensor showed a sensitivity of 1.02 mA/M cm 2 , and a very low detection limit of 5 μM. The electrode retained 97% of its initial electrocatalytic activity after 30 days of storage at 4 0 C in 50 mM sodium phosphate buffer, pH 7.0.