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Determination of Total Reduced Thiol Levels in Plasma Using a Bromide Substituted Quinone
Author(s) -
Lobo GraceAnn M.,
Chitre Sneha A.,
Rathod Spandan M.,
Smith Robert B.,
Leslie Ray,
Livingstone Callum,
Davis James
Publication year - 2007
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200703977
Subject(s) - thiol , bromide , chemistry , cysteine , glutathione , quinone , redox , human plasma , chromatography , combinatorial chemistry , inorganic chemistry , organic chemistry , enzyme
Abstract The exploitation of 2‐bromo‐1,4‐naphthoquinone (NQBr) as a selective redox label for the determination of reduced thiol functionalities (RSH) has been investigated. The system is selective for RSH functionality, giving distinct voltammetric signals for glutathione and cysteine but can also be adapted for broad spectrum thiol detection. Ion chromatographic protocols based on conductimetric detection enable total RSH analysis (protein and monomolecular moieties) within human plasma. Bromide released through the reaction can be easily quantified and integrated within normal IC measurements. The efficacy of the approach has been assessed and the response validated through comparison with the standard colorimetric technique.