Premium
Polyphenothiazine Modified Electrochemical Aptasensor for Detection of Human α‐Thrombin
Author(s) -
Porfirieva Anna,
Evtugyn Gennady,
Hianik Tibor
Publication year - 2007
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200703963
Subject(s) - aptamer , thrombin , methylene blue , human serum albumin , analyte , chemistry , phenothiazine , quartz crystal microbalance , chromatography , electrode , combinatorial chemistry , adsorption , nanotechnology , materials science , platelet , microbiology and biotechnology , organic chemistry , medicine , photocatalysis , immunology , pharmacology , biology , catalysis
QCM aptasensor for detection human thrombin has been developed on the base of polymeric forms of phenothiazine dyes, Methylene Blue and Methylene Green. Electrostatic accumulation of the analyte in the polyphenothiazine layer made it possible to increase the sensitivity of QCM detection of thrombin in comparison with bare gold electrodes coated with avidin or neutravidin. The influence of nonspecific binding of human serum albumin and the optimal composition of the surface layers were determined. The aptasensors developed make it possible to detect 10–100 nM of thrombin.