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Amperometric Glucose Biosensor Based on Glucose Oxidase Encapsulated in Carbon Nanotube–Titania–Nafion Composite Film on Platinized Glassy Carbon Electrode
Author(s) -
Choi Han Nim,
Han Jee Hoon,
Park Ji Ae,
Lee Joong Min,
Lee WonYong
Publication year - 2007
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200703958
Subject(s) - biosensor , glucose oxidase , nafion , ascorbic acid , carbon nanotube , hydrogen peroxide , amperometry , inorganic chemistry , materials science , glassy carbon , platinum nanoparticles , electrode , electrocatalyst , chemical engineering , nuclear chemistry , platinum , chemistry , catalysis , nanotechnology , cyclic voltammetry , organic chemistry , electrochemistry , food science , engineering
A highly sensitive and selective glucose biosensor has been developed based on immobilization of glucose oxidase within mesoporous carbon nanotube–titania–Nafion composite film coated on a platinized glassy carbon electrode. Synergistic electrocatalytic activity of carbon nanotubes and electrodeposited platinum nanoparticles on electrode surface resulted in an efficient reduction of hydrogen peroxide, allowing the sensitive and selective quantitation of glucose by the direct reduction of enzymatically‐liberated hydrogen peroxide at −0.1 V versus Ag/AgCl (3 M NaCl) without a mediator. The present biosensor responded linearly to glucose in the wide concentration range from 5.0×10 −5 to 5.0×10 −3 M with a good sensitivity of 154 mA M −1 cm −2 . Due to the mesoporous nature of CNT–titania–Nafion composite film, the present biosensor exhibited very fast response time within 2 s. In addition, the present biosensor did not show any interference from large excess of ascorbic acid and uric acid.