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Electrochemical Oxidation of ds‐DNA on Polypyrrole Nanofiber Modified Pencil Graphite Electrode
Author(s) -
Özcan Ali,
Şahin Yücel,
Özsöz Mehmet,
Turan Servet
Publication year - 2007
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200703957
Subject(s) - guanine , electrode , electrochemistry , polypyrrole , methylene blue , graphite , nuclear chemistry , nanofiber , dna , chemistry , materials science , inorganic chemistry , nanotechnology , organic chemistry , biochemistry , nucleotide , photocatalysis , gene , catalysis
Preparation of a polypyrrole nanofiber (PPyNF) modified pencil graphite (PG) electrode and its usage in the electrochemical DNA biosensors was investigated. The electrodes (PPyNF/PG1 and PPyNF/PG2) were prepared from a solution containing 0.1 M pyrrole, 0.1 M Na 2 CO 3 and 0.1 M LiCIO 4 by using potentiostatic and potentiodynamic methods. PPyNF/PG2 electrodes which were prepared by potentiostatic procedure showed higher responses for the oxidation of ds‐DNA than the PPyNF/PG1 electrodes prepared by potentiodynamic methods. Immobilization of the ds‐DNA on PG and PPyNF/PG surfaces was performed at a constant potential, +0.5 V, for 300 s in 0.5 M ABS (pH 4.8) containing 15 μg mL −1 ds‐DNA and 20 mM LiCIO 4 . The oxidation peak potentials of the ds‐DNA bases, guanine and adenine, were shifted to more cathodic values by using PPyNF/PG electrodes. The oxidation signal of the guanine base of ds‐DNA was decreased in the presence of methylene blue.