Fabrication of Active Horseradish Peroxidase Micropatterns with a High Resolution by Scanning Electrochemical Microscopy | Zendy

Li Xuemei | Zendy; Geng Qiaohong | Zendy; Wang Yingying | Zendy; Si Zhikun | Zendy; Jiang Wei | Zendy; Zhang Xiaoli | Zendy; Jin Wenrui | Zendy

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Fabrication of Active Horseradish Peroxidase Micropatterns with a High Resolution by Scanning Electrochemical Microscopy

Author(s) -

Li Xuemei,

Geng Qiaohong,

Wang Yingying,

Si Zhikun,

Jiang Wei,

Zhang Xiaoli,

Jin Wenrui

Publication year - 2007

Publication title -

electroanalysis

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.574

H-Index - 128

eISSN - 1521-4109

pISSN - 1040-0397

DOI - 10.1002/elan.200703928

Subject(s) - scanning electrochemical microscopy , horseradish peroxidase , electrochemistry , chemistry , electrode , materials science , substrate (aquarium) , resolution (logic) , analytical chemistry (journal) , nuclear chemistry , chromatography , organic chemistry , enzyme , oceanography , artificial intelligence , computer science , geology

We used a new reactive species OH − to fabricate active horseradish peroxidase (HRP) micropatterns with a high resolution by scanning electrochemical microscopy (SECM) coupled with a carbon fiber disk electrode as the SECM tip. In this method, except for active HRP micropatterns predesigned other regions on a HRP‐immobilized substrate were deactivated by OH − generated at the tip held at −1.7 V in 1.0 mol/L KCl containing 2.0×10 −3 mol/L benzoquinone (BQ) (pH 8.0). The feedback mode of SECM with a tip potential of −0.2 V was used to characterize the active HRP micropatterns in 1.0 mol/L KCl containing 2.0×10 −3 mol/L BQ and 2.0×10 −3 mol/L H 2 O 2 .

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