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Fabrication of Active Horseradish Peroxidase Micropatterns with a High Resolution by Scanning Electrochemical Microscopy
Author(s) -
Li Xuemei,
Geng Qiaohong,
Wang Yingying,
Si Zhikun,
Jiang Wei,
Zhang Xiaoli,
Jin Wenrui
Publication year - 2007
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200703928
Subject(s) - scanning electrochemical microscopy , horseradish peroxidase , electrochemistry , chemistry , electrode , materials science , substrate (aquarium) , resolution (logic) , analytical chemistry (journal) , nuclear chemistry , chromatography , organic chemistry , enzyme , oceanography , artificial intelligence , computer science , geology
We used a new reactive species OH − to fabricate active horseradish peroxidase (HRP) micropatterns with a high resolution by scanning electrochemical microscopy (SECM) coupled with a carbon fiber disk electrode as the SECM tip. In this method, except for active HRP micropatterns predesigned other regions on a HRP‐immobilized substrate were deactivated by OH − generated at the tip held at −1.7 V in 1.0 mol/L KCl containing 2.0×10 −3 mol/L benzoquinone (BQ) (pH 8.0). The feedback mode of SECM with a tip potential of −0.2 V was used to characterize the active HRP micropatterns in 1.0 mol/L KCl containing 2.0×10 −3 mol/L BQ and 2.0×10 −3 mol/L H 2 O 2 .