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Properties of Dendrimer‐Encapsulated Pt Nanoparticles Doped Polypyrrole Composite Films and Their Electrocatalytic Activity for Glucose Oxidation
Author(s) -
Tang Longhua,
Zhu Yihua,
Xu Lihuan,
Yang Xiaoling,
Li Chunzhong
Publication year - 2007
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200703904
Subject(s) - polypyrrole , glucose oxidase , amperometry , cyclic voltammetry , biosensor , materials science , platinum nanoparticles , dendrimer , chemical engineering , nanoparticle , conductive polymer , nanocomposite , dielectric spectroscopy , dispersity , electrochemistry , nanotechnology , polymer , polymer chemistry , chemistry , electrode , polymerization , composite material , engineering
A type of novel electroanalytical sensing nanobiocomposite material was prepared by electropolymerization of pyrrole containing poly(amidoamine) dendrimers‐encapsulated platinum nanoparticles (Pt‐PAMAM), and glucose oxidase (GOx). The Pt nanoparticles encapsulated in PAMAM are nearly monodisperse with an average diameter of 3 nm, and they provide electrical conductivity. Polypyrrole acts as a polymer backbone to give stable and homogeneous cast thin films, and it also defines the electrical conductivity. Both Polypyrrole and PAMAM can provide a favorable microenvironment to keep the bioactivity of enzymes such as glucose oxidase. The homogeneity of GOx/Pt‐PAMAM‐PPy nanobiocomposite films was characterized by atomic force microscopy (AFM). Amperometric biosensors fabricated with these materials were characterized electrochemically using cyclic voltammetry (CV), electrochemical impedance spectra (EIS) and amperometric measurements in the presence of hydrogen peroxide or glucose. All those show the resultant biosensor sensitivity was strongly enhanced within the nanobiocomposite film. The optimized glucose biosensor displayed a sensitivity of 164 μA mM −1 cm −1 , a linear range of 0.2 to 600 μM, a detection limit of 10 nM, and a response time of <3 s.