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Electrochemical Nucleic Acid Biosensors for the Detection of Interaction Between Peroxynitrite and DNA
Author(s) -
de la Fuente Elizabeth,
Villagra Gonzalo,
Bollo Soledad
Publication year - 2007
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200703885
Subject(s) - peroxynitrite , guanine , chemistry , nucleic acid , dna , oligonucleotide , biosensor , electrochemistry , biochemistry , combinatorial chemistry , superoxide , electrode , nucleotide , enzyme , gene
We studied the reactivity of peroxynitrite and different nucleic acid molecules using DNA electrochemical biosensors. SIN‐1 (3‐morpholinosydnonimine) has been used for the simultaneous generation of NO⋅and superoxide, i.e., as a peroxynitrite (ONOO − ) donor. Double strand DNA (dsDNA), single strand DNA (ssDNA) and 15 guanine bases oligonucleotide (Oligo(dG) 15 ) were immobilized on a carbon paste electrode to generate the biosensor and DPV was selected as the electroanalytical technique. Results showed that electrochemical biosensors were very sensitive for detecting interaction between ONOO − and DNA. A down/up effect was observed, i.e., at low ONOO − concentrations the guanine oxidation signal decreased while at high ONOO − concentrations the guanine oxidation current increased. Oligo(dG) 15 exhibited greater interaction at low ONOO − concentrations than the other DNA molecules. The reactivity between ONOO − and DNA was also evaluated in solution phase, showing the same down/up effect. Finally, the capacity of DNA to hybridize was prevented after interaction with ONOO − .