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A Reagentless Amperometric Immunosensor for Alpha‐Fetoprotein Based on Gold Nanoparticles/TiO 2 Colloids/Prussian Blue Modified Platinum Electrode
Author(s) -
Yuan Yanru,
Yuan Ruo,
Chai Yaqin,
Zhuo Ying,
Shi Yintao,
He Xiulan,
Miao Xiangmin
Publication year - 2007
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200603868
Subject(s) - prussian blue , amperometry , detection limit , platinum nanoparticles , electrode , platinum , colloidal gold , selectivity , chemistry , adsorption , chromatography , repeatability , nuclear chemistry , nanoparticle , materials science , electrochemistry , nanotechnology , catalysis , organic chemistry , biochemistry
A novel reagentless amperometric immunosensor for the determination of alpha‐fetoprotein (AFP) was prepared by immobilizing TiO 2 colloids on Prussian blue (PB) modified platinum electrode, which yielded a positively charged interface with strong adsorption to deposit gold nanoparticles for immobilization of alpha‐fetoprotein antibody (anti‐AFP). The factors influencing the performance of the proposed immunosensors were studied in detail. Under the optimized conditions, cyclic voltammograms determination of AFP showed a specific response in two concentration ranges from 3.0 to 30.0 ng/mL and from 30.0 to 300.0 ng/mL with a detection limit of 1.0 ng/mL at a signal‐to‐noise ratio of 3. The proposed immunosensor exhibited high selectivity, good reproducibility, long‐term stability (>2 months) and good repeatability.