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Amperometric Glucose Biosensing of Gold Nanoparticles and Carbon Nanotube Multilayer Membranes
Author(s) -
Liu Ying,
Wu Shuo,
Ju Huangxian,
Xu Li
Publication year - 2007
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200603814
Subject(s) - glucose oxidase , biosensor , amperometry , carbon nanotube , membrane , colloidal gold , nanoparticle , chemical engineering , materials science , nanotechnology , scanning electron microscope , electrochemistry , detection limit , chemistry , analytical chemistry (journal) , electrode , chromatography , composite material , biochemistry , engineering
A novel multilayer gold nanoparticles/multiwalled carbon nanotubes/glucose oxidase membrane was prepared by electrostatic assembly using positively charged poly(dimethyldiallylammonium chloride) to connect them layer by layer. The modification process and membrane structures were characterized by atomic force microscopy, scanning electron microscopy and electrochemical methods. This membrane showed excellent electrocatalytic character for glucose biosensing at a relatively low potential (−0.2 V). The K m value of the immobilized glucose oxidase was 10.6 mM. This resulting sensor could detect glucose up to 9.0 mM with a detection limit of 128 μM and showed excellent analytical performance.