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Study on Direct Electrochemistry of Glucose Oxidase Stabilized by Cross‐Linking and Immobilized in Silica Nanoparticle Films
Author(s) -
Liu Hongyun,
Hu Naifei
Publication year - 2007
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200603792
Subject(s) - glucose oxidase , pyrolytic carbon , glutaraldehyde , nanoparticle , highly oriented pyrolytic graphite , scanning electron microscope , materials science , electrochemistry , chemical engineering , electrode , chemistry , analytical chemistry (journal) , biosensor , nanotechnology , organic chemistry , scanning tunneling microscope , composite material , pyrolysis , engineering
Glucose oxidase (GOD) in SiO 2 nanoparticle films was cross‐linked by glutaraldehyde (GA), forming GOD‐GA‐SiO 2 films on the surface of pyrolytic graphite (PG) electrodes. GOD in the GOD‐GA‐SiO 2 films showed a pair of well defined and quasi‐reversible cyclic voltammetric peaks at −0.48 V vs. SCE in pH 7.0 buffers. Scanning electron microscopy of the films demonstrated that the interaction between GOD and GA/SiO 2 would make the morphology of dry GOD‐GA‐SiO 2 films very different from that of SiO 2 and GOD‐SiO 2 films. IR spectroscopy confirms that the GODs in the films are indeed cross‐linked through GA, and GA in the films greatly improves the film stability. UV‐vis and IR spectroscopy suggest that the enzyme is not denatured by cross‐linking in the films. The comparative bioelectrocatalytic study with free flavin adenine dinucleotide (FAD) in SiO 2 films indicates that GOD in the films retains its biocatalytic activity toward glucose oxidation. All these results show that SiO 2 nanoparticle films not only provide a favorable microenvironment for the native GOD to exchange electrons directly with underlying electrodes, but also supply a matrix for stabilizing GOD when combined with GA.