Premium
Carbon Nanotube Composite as Novel Platform for Microbial Biosensor
Author(s) -
Kirgoz Ülkü A.,
Timur Suna,
Odaci Dilek,
Pérez Briza,
Alegret Salvador,
Merkoçi Arben
Publication year - 2007
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200603786
Subject(s) - biosensor , electrode , glutaraldehyde , carbon nanotube , composite number , materials science , pseudomonas fluorescens , response surface methodology , graphite , central composite design , substrate (aquarium) , gelatin , chemical engineering , microbial fuel cell , nanotechnology , composite material , chemistry , chromatography , organic chemistry , bacteria , oceanography , anode , biology , geology , engineering , genetics
The presented work includes the development of a microbial biosensor based on a carbon‐nanotube epoxy composite (CNTEC) platform used as supporting electrode for cell immobilization. For this purpose, cells of Pseudomonas fluorescens were immobilized on the surface of the CNTEC electrode by means of gelatin which was then cross linked with glutaraldehyde. After optimization of experimental parameters like cell amount, pH and temperature, the system was calibrated for glucose. From the calibration graph the linear range was estimated as 0.5–4.0 mM with a response time of 100 s. Furthermore, substrate specificity and operational stability were investigated. Finally, the results that were obtained with CNTEC electrodes were compared with conventional graphite epoxy composite electrode (GECE) and as a result, higher current values (2 to 3 folds) were observed with CNTEC microbial biosensor.