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Single‐Walled Carbon Nanotube Based Real‐Time Organophosphate Detector
Author(s) -
Liu Ningyi,
Cai Xianpeng,
Lei Yu,
Zhang Qing,
ChanPark Mary B.,
Li Changming,
Chen Wilfred,
Mulchandani Ashok
Publication year - 2007
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200603761
Subject(s) - organophosphate , carbon nanotube , paraoxon , biosensor , conductance , nanotechnology , materials science , dielectrophoresis , hydrolysis , chemistry , organic chemistry , microfluidics , acetylcholinesterase , enzyme , pesticide , mathematics , combinatorics , agronomy , biology
A novel single‐walled carbon nanotube (SWNT) based biosensor for real‐time detection of organophosphate has been developed. Horizontally aligned SWNTs are assembled to desirable electrodes using AC dielectrophoresis technique. Organophosphorus hydrolase (OPH) immobilized on the SWNTs by nonspecific binding triggers enzymatic hydrolysis of organophosphates (OPs), such as paraoxon, consequently causing a detectable change in the conductance of the SWNTs. The conductance change is found to be correlated to the concentration of organophosphate. Our results suggest the novel biosensor has great potential to serve as a simple and reusable platform of sensing organophosphate on a real‐time basis.