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Rutin Determination at an Amperometric Biosensor
Author(s) -
Mousty Christine,
Cosnier Serge,
SanchezPaniagua Lopez Marta,
LopezCabarcos Enrique,
LopezRuiz Beatriz
Publication year - 2007
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200603718
Subject(s) - biosensor , rutin , amperometry , chemistry , chromatography , redox , matrix (chemical analysis) , electrode , electrochemistry , materials science , inorganic chemistry , organic chemistry , biochemistry , antioxidant
The feasibility of amperometric detection of rutin at a biosensor using polyphenol oxidase (PPO) as bioelement was investigated. The biosensor was fabricated by entrapment of PPO within a laponite clay film coated on an electrode surface. The amperometric detection of rutin was carried out at −0.1 V via the direct electrochemical reduction of the product of the enzymatic reaction or its mediated reduction by a phenothiazine redox mediator (Azure B) intercalated within the clay matrix. The biosensor response was optimized with regard to pH, temperature, applied potential ( E app ) , and clay film thickness. The biosensor performance was discussed depending on the detection principle.