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Flow Injection Analysis of Chloramphenicol by Using a Disposable Wall‐Jet Ring Disk Carbon Electrode
Author(s) -
Liao ChunYen,
Chang ChiaChen,
Ay Chyung,
Zen JyhMyng
Publication year - 2007
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200603706
Subject(s) - detection limit , electrode , flow injection analysis , reproducibility , chemistry , hydroxylamine , overpotential , aqueous solution , analytical chemistry (journal) , standard hydrogen electrode , electrochemistry , reference electrode , chromatography , organic chemistry
A preanodized screen‐printed ring disk carbon electrode was applied to the determination of chloramphenicol (PhNO 2 , CAP) by flow injection analysis (FIA). By setting up the first irreversible reduction reaction of PhNO 2 to PhNHOH at the disk electrode, the following reversible oxidation of hydroxylamine (PhNHOH) to the nitroso (PhNO) derivative can be monitored/collected at the ring electrode for CAP analysis. The interference from dissolved oxygen and others can thus be avoided by using this approach and precise CAP determination can be easily performed by FIA under aerobic conditions. Preanodization treatment helps to lower the overpotential of the electrochemical reaction of CAP and favors the selective detection in aqueous medium. Under the optimum conditions, ten repetitive determinations at 1 μM and 10 μM CAP resulted in relative standard deviations of less than 4%, indicating good reproducibility of the system. A linear calibration range of 0.1–20 μM with a detection limit of 0.074 μM ( S / N =3) was obtained. Veterinary pharmaceutics were finally analyzed by this sensor to validate its practical applicability.