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Preconcentration and Determination of a Phospholipid at a Surface Modified by Layer‐by‐Layer Assembly
Author(s) -
Wandstrat M. M.,
Cox J. A.
Publication year - 2007
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200603684
Subject(s) - quartz crystal microbalance , electrochemistry , layer by layer , layer (electronics) , chemistry , dendrimer , bilayer , electrode , catalysis , chemical engineering , analytical chemistry (journal) , inorganic chemistry , adsorption , membrane , chromatography , polymer chemistry , organic chemistry , biochemistry , engineering
Electrochemical oxidation of a phospholipid, phosphatidylcholine (PC), was accomplished at a 4‐aminothiophenol (ATP)‐modified gold electrode coated with a layer‐by‐layer assembly of an electrochemical catalyst (dirhodium phosphomolybdic acid), a trapping agent for PC (a cyclophane, CP, derivative, 1,4‐xylylene‐1,4‐phenylene‐diacetate), and a spacer (generation‐4 polyamidoamine dendrimer, PAMAM). The layer‐by‐layer assembly process and the trapping of PC was verified by quartz crystal microbalance measurements; Au|ATP|CP|PAMAM|CP trapped (1.5±0.4)×10 −9 mol cm −2 of PC. The electrocatalytic oxidation of PC yielded a current that varied linearly with concentration over the range 1–50 μM; the R 2 value was 0.996.