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Nanoarray‐Surfaces by Reconstitution of the Porin MspA into Stabilized Long‐Chain‐Lipid‐Monolayers at a Gold‐Surface
Author(s) -
Wörner Michael,
Niebler Sandra,
Gogritchiani Eliso,
Egner Nicole,
Braun André M.,
Niederweis Michael,
Bossmann Stefan H.
Publication year - 2006
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200603636
Subject(s) - monolayer , cyclic voltammetry , chemistry , colloidal gold , copper , self assembled monolayer , electrode , dielectric spectroscopy , lipid bilayer , membrane , electrochemistry , photochemistry , materials science , nanoparticle , nanotechnology , organic chemistry , biochemistry
We have reconstituted the channel porin MspA from M. smegmatis on a gold electrode into a long‐chain lipid monolayer resembling a biological membrane. The tailored deposition of the self‐assembled monolayer (SAM) on gold was achieved by employing electrochemically initiated generation of thiyl‐radicals from organic thiosulfates (Bunte‐Salts), which then adsorb onto the gold's surface. At surface coverage of the lipids of approximately 80%, MspA reconstituted efficiently from the aqueous buffer into the SAM. The combination of cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), reflection electron microscopy (REM) and the formation of copper(0)‐nanoparticles from copper(II)‐ions inside the MspA‐channels proved that MspA forms SAM‐spanning ion channels on gold, keeping the electrode (surface) accessible through the pores.