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Real‐Time PCR Sensing by Thermal Conductivity Monitoring
Author(s) -
Noh Seungbeom,
Lee Jinsang,
Shin JangKyoo,
Lim Geunbae
Publication year - 2006
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200603622
Subject(s) - real time polymerase chain reaction , thermal conductivity , polymerase chain reaction , dna , transillumination , conductivity , sample (material) , primer dimer , computer science , analytical chemistry (journal) , biological system , materials science , computational biology , chemistry , chromatography , biology , physics , biochemistry , multiplex polymerase chain reaction , gene , optics , composite material
Traditional PCR (Polymerase Chain Reaction) technology is based on an end‐point system which confirms the existence of a target DNA sequence by electrophoresis and UV transillumination steps. This process is labor‐intensive and time consuming. A real‐time PCR is the best alternative that can improve problems of analysis time and accuracy. Therefore, we propose a smaller, non‐optical and non‐labeling system with the advantages of an established real‐time PCR. The proposed technique utilizes a variation of the thermal conductivity of the DNA sample with a PCR cycle. We confirmed that thermal conductivity depends on amounts of PCR products.