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Development of Quantum Dots Modified Acetylcholinesterase Biosensor for the Detection of Trichlorfon
Author(s) -
Li XiaoHua,
Xie Zonghong,
Min Hong,
Li Chenxin,
Liu Meichuan,
Xian Yuezhong,
Jin Litong
Publication year - 2006
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200603615
Subject(s) - biosensor , acetylcholinesterase , organophosphate , chemistry , electrochemistry , nuclear chemistry , detection limit , combinatorial chemistry , chromatography , nanotechnology , pesticide , enzyme , organic chemistry , materials science , electrode , biochemistry , biology , agronomy
Poly ( N ‐vinyl‐2‐pyrrolidone) (PVP)‐capped CdS quantum dots (QCdS‐PVP) was synthesized with CdCl 2 and Na 2 S in the presence of PVP. QCdS‐PVP has been used for the immobilization and stabilization of the acetylcholinesterase (AChE). The electrocatalytic activity of QCdS‐PVP leads to a greatly improved electrochemical detection of the enzymatically generated thiocholine product, and higher sensitivity and stability. The GCE/QCdS‐PVP/AChE biosensor was used for the detection of organophosphate pesticides (OPs), such as trichlorfon. The sensor performance, including pH and inhibition time, was optimized with respect to operating conditions. Under the optimal conditions, the biosensor was used to measure as low as 12 ppb trichlorfon with a 5‐min inhibition time.