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DNA Biosensor Concepts Based on a Change in the DNA Persistence Length upon Hybridization
Author(s) -
Mearns Freya J.,
Wong Elicia L. S.,
Short Ken,
Hibbert D. Brynn,
Gooding J. Justin
Publication year - 2006
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200603614
Subject(s) - biosensor , dna , dna–dna hybridization , flexibility (engineering) , nanotechnology , ferrocene , materials science , hybridization probe , nucleic acid thermodynamics , chemistry , biophysics , electrochemistry , electrode , biology , biochemistry , base sequence , statistics , mathematics
Hybridization‐induced physical changes of DNA are exploited in the development of DNA switchable surfaces for electrochemical biosensing purposes. Two types of biosensing concepts are explored, both based on the same basic switchable surface. The interface is designed so that the end‐tethered DNA is able to switch from a flexible state to a rigid one upon hybridization. The first biosensing concept described is a label‐free system that uses air oxidation of the interface, followed by the change in accessibility of the surface upon hybridization to detect complementary target DNA. The second is a ferrocene‐labeled system exploiting the change in DNA flexibility alone. Atomic force microscopy studies of the DNA switching surface are described.