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Bioelectrocatalytic Oxidation of Glucose by Immobilized Bacteria Gluconobacter oxydans. Evaluation of Water‐Insoluble Mediator Efficiency
Author(s) -
Babkina Elena,
Chigrinova Ekaterina,
Ponamoreva Ol'ga,
Alferov Valery,
Reshetilov Anatoly
Publication year - 2006
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200603608
Subject(s) - mediator , electron acceptor , substrate (aquarium) , chemistry , benzoquinone , catalysis , bacteria , ferrocene , acceptor , electrochemistry , combinatorial chemistry , electrode , biochemistry , biology , ecology , genetics , endocrinology , physics , condensed matter physics
For the first time it has been shown possible to use strain G. oxydans sbsp. industrius VKM B‐1280 in combination with water‐insoluble mediators: ferrocene, 1,1′‐dimethylferrocene, 2,5‐dibromo‐1,4‐benzoquinone, and 2‐methyl‐1,4‐benzoquinone in the process of electrocatalytic oxidation of glucose. Cell‐mediator interaction has been studied using a carbon paste electrode that comprised a mediator and immobilized bacteria. Catalytic activity of the system “mediator – bacterial cell – substrate” was characterized by three quantities: the maximum reaction rate and the ratios of the Michaelis constant to the distribution constant for the substrate and to that for the electron acceptor. It has been revealed that 2,5‐dibromo‐1,4‐benzoquinone is the most efficient for high catalytic activity.