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Site‐Directed Immobilization of Proteins Through Electrochemical Deprotection on Electroactive Self‐Assembled Monolayers
Author(s) -
Dondapati Srujan Kumar,
Montornes Josep M.,
Sanchez Pablo Lozano,
Sanchez Josep Lluis Acero,
O'Sullivan Ciara,
Katakis Ioanis
Publication year - 2006
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200603606
Subject(s) - chemistry , self assembled monolayer , electrochemistry , horseradish peroxidase , moiety , glucose oxidase , cyclic voltammetry , covalent bond , surface plasmon resonance , amperometry , electrode , monolayer , combinatorial chemistry , substrate (aquarium) , selectivity , biosensor , organic chemistry , nanotechnology , enzyme , materials science , nanoparticle , catalysis , biochemistry , oceanography , geology
Self‐assembled monolayers (SAM) were obtained on gold electrodes using thioctic esters of benzo[1,3]dioxinol. These SAMs present a group that can be electroactivated selectively and was used for the directed, reagentless, covalent patterning of proteins. The advantage of this moiety is that it allows electroactivation at low potentials increasing selectivity and reliability. In this study, the efficiency of this patterning system is examined. Cyclic voltammetry (CV) was used to confirm the electroactive nature of SAM modified electrodes, showing fast and complete electrochemical deprotection with one scan. The enzymes glucose oxidase (GOx) and horseradish peroxidase (HRP) were patterned on the SAM‐modified electrode through Schiff's base formation after electrochemical deprotection, confirming the selective nature of the electroactive substrate. Amperometric response was measured after the GOx immobilization showing high selective response. Real time monitoring is shown by immobilization of HRP on the SAM modified surface using electrochemical surface plasmon resonance (ESPR) after electrochemical deprotection, again showing high selective response when compared to the protected control.