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Electrically Addressed Fabrication of Aptamer‐Based Array Electrodes
Author(s) -
Xu Dawei,
Han Huanhuan,
He Wei,
Liu Zhihong,
Xu Danke,
Liu Xijie
Publication year - 2006
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200603593
Subject(s) - aptamer , electrode , fabrication , materials science , nanotechnology , optoelectronics , chemistry , medicine , genetics , alternative medicine , pathology , biology
An aptamer immobilization method based electrically addressed fabrication has been developed for the preparation of aptamer‐modified arrayed electrodes, by which the human IgE aptamer was oriented and immobilized on the gold electrode surface. The optimization of the experimental conditions including the applied potential, time and scan rate of potential was investigated. The method was successfully used to immobilize the aptamer onto the desired electrodes, pixel by pixel, based on the electrically addressed approach. Compared to the control electrodes, the resulting aptamer‐modified electrodes showed their specific recognition for human IgE. The present method owns several advantages such as rapid and simple immobilization as well as its automatic addressed capability by the electric approach.