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Gold Nanoparticles Doped Three‐Dimensional Sol‐gel Matrix for Amperometric Human Chorionic Gonadotrophin Immunosensor
Author(s) -
Chen Jin,
Yan Feng,
Tan Fang,
Ju Huangxian
Publication year - 2006
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200603575
Subject(s) - amperometry , nanoparticle , colloidal gold , biocompatibility , matrix (chemical analysis) , nuclear chemistry , immunoassay , chemistry , chromatography , analyte , immunoconjugate , materials science , electrochemistry , nanotechnology , electrode , organic chemistry , monoclonal antibody , antibody , immunology , biology
A novel electrochemical immunosensor for human chorionic gonadotrophin (hCG) was proposed by immobilization of hCG in gold nanoparticles doped three‐dimensional (3D) sol‐gel matrix and an interfacial competitive immunoreaction. The 3D organized composite structure was prepared by assemble of gold nanoparticles into a hydrolyzed (3‐mercaptopropyl)‐trimethoxysilane sol‐gel matrix, which showed good biocompatibility. After the interfacial competitive immunoreaction the formed HRP‐labeled immunoconjugate showed good enzymatic activity for the oxidation of o ‐phenylenediamine by H 2 O 2 . With a competitive format, a method comprising of o ‐phenylenediamine‐H 2 O 2 ‐immobilized HRP labeled hCG immunoconjugate system for immunoassay of hCG from 5.0 to 30.0 mIU mL −1 was developed. The immunosensor showed good precision, high sensitivity, acceptable stability and reproducibility and could be used for detection of hCG in human serum with the consistent results in comparison with those obtained by a commercial analyzer.