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Electrochemical Studies of Oxidation of Lomefloxacin and Interaction with Calf Thymus DNA at Nano‐SnO 2 /DHP Modified Electrode
Author(s) -
Zhang Yuzhong,
Cai Yuejuan,
Su Shao,
Ni Yonghong
Publication year - 2006
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200603543
Subject(s) - electrode , cyclic voltammetry , electrochemistry , chemistry , lomefloxacin , absorbance , analytical chemistry (journal) , glassy carbon , buffer solution , adduct , inorganic chemistry , nuclear chemistry , chromatography , organic chemistry , biochemistry , norfloxacin , ciprofloxacin , antibiotics
A nanoparticle thin film modified electrode has been constructed using a glassy carbon electrode (GCE) coated with a nano‐tin oxide/dihexadecylphosphate (nano‐SnO 2 /DHP). In pH 6.0 phosphate buffer solutions (PBS), lomefloxacin (LMF) appeared as an anodic peak with peak potential of 1.35 V at nano‐SnO 2 /DHP modified electrode. In comparison with a bare GCE or a nano‐SnO 2 modified electrode, the nano‐SnO 2 /DHP modified electrode exhibited an enhanced effectiveness for the oxidation of LMF. Cyclic voltammetry (CVs) coupled with fluorescence and UV/vis absorbance spectra techniques were used to study the interaction of LMF with Calf thymus DNA (ctDNA) in phosphate buffer solutions (PBS). The interaction of LMF and ctDNA could result in a considerable decrease in the peak currents and positive shift in the peak potential, as well as changes of fluorescence, UV/vis adsorption spectra and gel electrophoresis. All the acquired data showed that the new adduct between LMF and ctDNA was formed. Electrochemistry coupled with spectroscopy techniques could provide a relatively easy way to obtain useful information about the molecular mechanism of LMF‐ctDNA interactions.